Молекулярная нейробиология и нейроонкология

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Руководитель

Каспаров Сергей Ашотович

ДМН, профессор молекулярной физиологии университета Бристоля

Телефон:  +44 (0) 117 331 2275

Почта: Sergey.Kasparov@bristol.ac.uk








Научные интересы и направления работы: 

  • Изучение сигнальных механизмов взаимодействия глии и нейронов, потенциальных точек приложения новых лекарственных препаратов а так же физиологических и патологических процессов с участием астроглии
  • Изучение подходов к молекулярной и генетической терапии глиобластомы – наиболее злокачественной опухоли центральной нервной системы
  • Создание новых молекулярных и генетических инструментов для изучения мозга и контроля сигнальных систем.

Цели:

  1. Внедрение в ИЖС современных методов исследования мозга, с использованием генной инженерии, имеджинга, оптогенетики, трансфера генов и формирование пула компетенции.
  2. Разработка новых подходов к терапии глиобластомы, основанных на комбинации молекулярной биологии и оптики.
  3. Выяснение роли определенных сигнальных систем, локализованных в астроцитах, в различных функциях мозга, например в обучении или контроле эмоций.
  4. Формировании культуры научных публикаций в цитируемых международных журналах

Примером нашей работы является видео-абстракт публикации, сделанной с участием аспирантки БФУ Д Прокудиной, опубликованной в журнале Glia (IF=5.8).


Команда

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Аспирант 3 года 

Васильев Александр

Тел. +7 (952) 791 23 93

OTHERLIFE@bk.ru   



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Аспирантка 2 года 

Прокудина Даша

Тел. +7 9211084462

DProkudina@stud.kantiana.ru



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Аспирант 2 года 

Можей Олег

Тел. +7 (958) 585 44 54

oleg.mozhei@bristol.ac.uk




Научно-исследовательские проекты группы




 

Публикации

Публикации руководителя и членов группы за 2013-2018г.

Кроме этого 2 абстракта опубликованы в сборнике конференции BIOINFORMATICS OF GENOME REGULATION AND STRUCTURE\SYSTEMS BIOLOGY (BGRS\SB-2018), Новосибирск.

1.                  Angelova PR, Iversen KZ, Teschemacher AG, Kasparov S, Gourine AV, & Abramov AY (2018). Signal transduction in astrocytes: Localization and release of inorganic polyphosphate. Glia.

2.                  Barros LF, Bolanos JP, Bonvento G, Bouzier-Sore AK, Brown A, Hirrlinger J, Kasparov S, Kirchhoff F, Murphy AN, Pellerin L, Robinson MB, & Weber B (2018). Current technical approaches to brain energy metabolism. Glia 66, 1138-1159.

3.                  Hosford PS, Mosienko V, Kishi K, Jurisic G, Seuwen K, Kinzel B, Ludwig MG, Wells JA, Christie IN, Koolen L, Abdala AP, Liu BH, Gourine AV, Teschemacher AG, & Kasparov S (2018). CNS distribution, signalling properties and central effects of G-protein coupled receptor 4. Neuropharmacology.

4.                  Liu B, Mosienko V, Vaccari CB, Prokudina D, Huentelman M, Teschemacher AG, & Kasparov S (2018). Glio- and neuro-protection by prosaposin is mediated by orphan G-protein coupled receptors GPR37L1 and GPR37. Glia.

5.                  McBryde FD, Liu BH, Roloff EV, Kasparov S, & Paton JFR (2018). Hypothalamic paraventricular nucleus neuronal nitric oxide synthase activity is a major determinant of renal sympathetic discharge in conscious Wistar rats. Exp Physiol 103, 419-428.

6.                  Rajani V, Zhang Y, Jalubula V, Rancic V, Sheikhbahaei S, Zwicker JD, Pagliardini S, Dickson CT, Ballanyi K, Kasparov S, Gourine AV, & Funk GD (2018). Release of ATP by pre-Botzinger complex astrocytes contributes to the hypoxic ventilatory response via a Ca(2+) -dependent P2Y1 receptor mechanism. J Physiol 596, 3245-3269.

7.                  Roloff EVL, Walas D, Moraes DJA, Kasparov S, & Paton JFR (2018). Differences in autonomic innervation to the vertebro-basilar arteries in spontaneously hypertensive and Wistar rats. J Physiol.

8.                  Sheikhbahaei S, Turovsky EA, Hosford PS, Hadjihambi A, Theparambil SM, Liu B, Marina N, Teschemacher AG, Kasparov S, Smith JC, & Gourine AV (2018). Astrocytes modulate brainstem respiratory rhythm-generating circuits and determine exercise capacity. Nat Commun 9, 370.

9.                  Vasilev A, Sofi R, Tong L, Teschemacher AG, & Kasparov S. In Search of a Breakthrough Therapy for Glioblastoma Multiforme. Neuroglia 1, 292-311. 2018.

10.             Barros LF, Bolanos JP, Bonvento G, Bouzier-Sore AK, Brown A, Hirrlinger J, Kasparov S, Kirchhoff F, Murphy AN, Pellerin L, Robinson MB, & Weber B (2017). Current technical approaches to brain energy metabolism. Glia 1-22.

11.             Benford H, Bolborea M, Pollatzek E, Lossow K, Hermans-Borgmeyer I, Liu B, Meyerhof W, Kasparov S, & Dale N (2017). A sweet taste receptor-dependent mechanism of glucosensing in hypothalamic tanycytes. Glia 65, 773-789.

12.             Christie IN, Wells JA, Kasparov S, Gourine AV, & Lythgoe MF (2017). Volumetric Spatial Correlations of Neurovascular Coupling Studied using Single Pulse Opto-fMRI. Sci Rep 7, 41583.

13.             Liu B, Teschemacher AG, & Kasparov S (2017a). Neuroprotective potential of astroglia. J Neurosci Res 95, 2126-2139.

14.             Liu B, Teschemacher AG, & Kasparov S (2017b). Astroglia as a cellular target for neuroprotection and treatment of neuro-psychiatric disorders. Glia 65, 1205-1226.

15.             Machhada A, Trapp S, Marina N, Stephens RCM, Whittle J, Lythgoe MF, Kasparov S, Ackland GL, & Gourine AV (2017). Vagal determinants of exercise capacity. Nat Commun 8, 15097.

16.             Mosienko V, Chang AJ, Alenina N, Teschemacher AG, & Kasparov S (2017). Rodents and humans are able to detect the odour of L-Lactate. PLoS ONE 12, e0178478.

17.             Rajani V, Zhang Y, Jalubula V, Rancic V, Sheikhbahaei S, Zwicker JD, Pagliardini S, Dickson CT, Ballanyi K, Kasparov S, Gourine AV, & Funk GD (2017). Release of ATP by pre-Botzinger complex astrocytes contributes to the hypoxic ventilatory response via a Ca2+ -dependent P2Y1 receptor mechanism. J Physiol.

18.             Karagiannis A, Sylantyev S, Hadjihambi A, Hosford PS, Kasparov S, & Gourine AV (2016). Hemichannel-mediated release of lactate. J Cereb Blood Flow Metab 36, 1202-1211.

19.             Kasparov S (2016). Are Astrocytes the Pressure-Reservoirs of Lactate in the Brain? Cell Metab 23, 1-2.

20.             Marina N, Teschemacher AG, Kasparov S, & Gourine AV (2016a). Glia, sympathetic activity and cardiovascular disease. Exp Physiol 101, 565-576.

21.             Marina N, Kasymov V, Ackland GL, Kasparov S, & Gourine AV (2016b). Astrocytes and Brain Hypoxia. Adv Exp Med Biol 903, 201-207.

22.             Mayorov D, Kasparov S, & Paton J (2016). OS 05-09 REDUCED VASODILATOR EFFICIENCY OF ADENOSINE IN THE BRAINSTEM OF YOUNG SPONTANEOUSLY HYPERTENSIVE RATS. J Hypertens 34 Suppl 1, e60.

23.             Roloff EV, Tomiak-Baquero AM, Kasparov S, & Paton JF (2016). Parasympathetic innervation of vertebro-basilar arteries: is this a potential clinical target? J Physiol 594, 6463-6585.

24.             Turovsky E, Theparambil SM, Kasymov V, Deitmer JW, Del Arroyo AG, Ackland GL, Corneveaux JJ, Allen AN, Huentelman MJ, Kasparov S, Marina N, & Gourine AV (2016). Mechanisms of CO2/H+ Sensitivity of Astrocytes. J Neurosci 36, 10750-10758.

25.             Angelova PR, Kasymov V, Christie I, Sheikhbahaei S, Turovsky E, Marina N, Korsak A, Zwicker J, Teschemacher AG, Ackland GL, Funk GD, Kasparov S, Abramov AY, & Gourine AV (2015). Functional Oxygen Sensitivity of Astrocytes. J Neurosci 35, 10460-10473.

26.             Hainer C, Mosienko V, Koutsikou S, Crook JJ, Gloss B, Kasparov S, Lumb BM, & Alenina N (2015). Beyond Gene Inactivation: Evolution of Tools for Analysis of Serotonergic Circuitry. ACS Chem Neurosci 6, 1116-1129.

27.             Kourtesis I, Kasparov S, Verkade P, & Teschemacher AG (2015). Ultrastructural Correlates of Enhanced Norepinephrine and Neuropeptide Y Cotransmission in the Spontaneously Hypertensive Rat Brain. ASN Neuro 7.

28.             Marina N, Ang R, Machhada A, Kasymov V, Karagiannis A, Hosford PS, Mosienko V, Teschemacher AG, Vihko P, Paton JF, Kasparov S, & Gourine AV (2015). Brainstem hypoxia contributes to the development of hypertension in the spontaneously hypertensive rat. Hypertension 65, 775-783.

29.             Mosienko V, Teschemacher AG, & Kasparov S (2015). Is L-lactate a novel signaling molecule in the brain? J Cereb Blood Flow Metab 35, 1069-1075.

30.             Teschemacher AG, Gourine AV, & Kasparov S (2015). A Role for Astrocytes in Sensing the Brain Microenvironment and Neuro-Metabolic Integration. Neurochem Res 40, 2386-2393.

31.             Wells JA, Christie IN, Hosford PS, Huckstepp RT, Angelova PR, Vihko P, Cork SC, Abramov AY, Teschemacher AG, Kasparov S, Lythgoe MF, & Gourine AV (2015). A critical role for purinergic signalling in the mechanisms underlying generation of BOLD fMRI responses. J Neurosci 35, 5284-5292.

32.             Figueiredo M, Lane S, Stout RF, Jr., Liu B, Parpura V, Teschemacher AG, & Kasparov S (2014). Comparative analysis of optogenetic actuators in cultured astrocytes. Cell Calcium 56, 208-214.

33.             Tang F, Lane S, Korsak A, Paton JF, Gourine AV, Kasparov S, & Teschemacher AG (2014). Lactate-mediated glia-neuronal signalling in the mammalian brain. Nat Commun 5, 3284.

34.             Hewinson J, Paton JF, & Kasparov S (2013). Viral gene delivery: optimized protocol for production of high titer lentiviral vectors. Methods Mol Biol 998, 65-75.

35.             Kasparov S & Herlitze S (2013). Optogenetics at a crossroads? Exp Physiol 98, 971-972.

36.             Kasymov V, Larina O, Castaldo C, Marina N, Patrushev M, Kasparov S, & Gourine AV (2013). Differential Sensitivity of Brainstem versus Cortical Astrocytes to Changes in pH Reveals Functional Regional Specialization of Astroglia. J Neurosci 33, 435-441.

37.             Marina N, Tang F, Figueiredo M, Mastitskaya S, Kasimov V, Mohamed-Ali V, Roloff E, Teschemacher AG, Gourine AV, & Kasparov S (2013). Purinergic signalling in the rostral ventro-lateral medulla controls sympathetic drive and contributes to the progression of heart failure following myocardial infarction in rats. Basic Res Cardiol 108, 317.

38.             Waki H, Hendy EB, Hindmarch CC, Gouraud S, Toward M, Kasparov S, Murphy D, & Paton JF (2013). Excessive leukotriene b4 in nucleus tractus solitarii is prohypertensive in spontaneously hypertensive rats. Hypertension 61, 194-201.












































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